• Special Bile
Description NZP Special Bile is a refined bile product containing the natural conjugated bile acids and is used as a selective inhibitory agent in microbiological media. It differs from Ox Bile Extract in several ways, thus is preferred for media such as MacConkey broth and agar, Brilliant Green Bile Broth and EE Broth.

The Bile used for Special Bile is carefully selected to contain very low levels (<0.5% w/w) of free deoxycholic acid. Bile powders with high levels of deoxycholic acid demonstrate a toxicity that may be unacceptable for use in, for example, MacConkey media and EE Broth.

The colour of Special Bile is carefully controlled in order to provide media suppliers with a consistent product for MacConkey and Brilliant Green Bile Broths, by being light enough in colour for effective and consistent dye coloration.

Unlike crude Bile Powders, the product will not form a surface scum or a deposit in culture media.
Use of Chemical Used in microbiological culture media. A major use is in clinical microbiology to selectively grow faecal staphylococci and streptococci (MacConkey Agar CM7/7b).

MacConkey Broth and Agar made with Special Bile at the Minimum Effective Concentration supports satisfactory growth of bile tolerant organisms, including pathogenic staphylococci. Gas production by clostridia is inhibited in broths containing higher concentrations of Special Bile (eg. Brilliant Green Bile Broth), therefore eliminating false positives for E. coli.

These are just two selected examples of the advantages seen by culture media manufacturers for the use of Special Bile in their selective media.
Solubility Water soluble
Standard Packsize 25 kg

Special Bile

  • Product Code: 22

Standard pack sizes are displayed in the product details. For non-standard pack sizes an additional repacking fee may apply.