• N-Acetyl-D-Mannosamine
Description N-Acetyl-D-mannosamine, manufactured in bulk (GMP) at NZP, is a ubiquitous but rare monosaccharide involved in a range of metabolic processes. It is often used as a precursor in the chemical or enzymatic synthesis of the neuraminic acids found in glycolipids and glycoproteins.

In biological systems it is the precursor compound for the biosynthesis of the sialic acids especially N-acetylneuraminic acid (Neu5Ac, NANA). For this reason N-acetyl-D-mannosamine is of keen interest to scientists around the world. NZP's N-acetyl-D-mannosamine is top grade material suitable for all purposes, biological or synthetic.
CAS Number 676347-48-1; (7772-94-3; 3615-17-6)
Formula

C8H15NO6.H2O

Molecular Weight 221.21 (Anhydrous)
Synonyms ManNAc; NAM; N-Acetylmannosamine monohydrate; DEX-M74
Use of Chemical N-Acetyl-D-mannosamine is becoming a favoured media component to enhance sialylation in CHO, BHK and HEK mammalian cell culture glycoprotein and mAb production

For application as a intermediate in chemical synthesis, please see N-acetyl-D-mannosamine (product code 75) which is better suited for these applications.
Relevant Publications Quantitative hydrophilic interaction chromatography–mass spectrometry analysis of N-acetylneuraminic acid and N-acetylmannosamine in human plasma
Yifan Shi, Xin Xu, Meng Fang, Michael Zhang, Yinghe Li, Brad Gillespie, Selwyn Yorke, Nora Yang, John C. McKew, William A Gahl, Marjan Huizing, Nuria Carrillo-Carrasco, Amy Qiu Wang
Highlights
  • HILIC LC MS/MS methods for ManNAc and Neu5Ac in human plasma are described.
  • A fit-for-purpose approach was adopted during method validation and sample analysis.
  • A surrogate matrix, 5% BSA, was utilized for the preparation of calibration standards.
  • Extraction with a phospholipid removal plate eliminated matrix effect.
Read the full article here.

"N-Acetylmannosamine and Mammalian Cell Culture Production of Recombinant Therapeutic Human Glycoproteins"
This expanded update of the mini-review originally published early in 2013, covers developments in the understanding, role and value of N-acetylmannosamine (ManNAc) to increase sialylation in the production of recombinant therapeutic human glycoproteins and monoclonal antibodies.
Read the review here


"The application of N-acetylmannosamine to the mammalian cell culture production of recombinant human glycoproteins."
This mini-review covers recent developments in the understanding, role and value of N-acetylmannosamine (ManNAc) as a compound that can improve sialylation during the production of recombinant human glycoproteins.
Read the review here

"Effects of Sugar Chain Precursors on Recombinant Protein Production in BHK Cells". Megumi Hayashi, Kaori Doi, Ichiro Ebata, Shinya Yamaguchi, Yasuhiro Ohta, Satoshi Terada, Animal Cell Technology: Basic & Applied Aspects, 2009, 15, 81-86.

“Improvement of interferon-gamma sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine”. Gu X, Wang DI. Biotechnol Bioeng., 1998, 58, (6), 642-8.
Appearance White powder
Solubility Soluble in water.
EC Number 616-491-5
MDL Number MFCD00046758
InChI OVRNDRQMDRJTHS-ZTVVOAFPSA-N
UNII X80PR7P73R
Standard Packsize 10 kg

N-Acetyl-D-Mannosamine

  • Product Code: 74

Standard pack sizes are displayed in the product description. For non-standard pack sizes an additional repacking fee may apply.