Structural Formula

N-Acetyl-D-mannosamine (ManNAc)



N-Acetyl-D-mannosamine (ManNAc)


N-Acetyl-D-mannosamine (ManNAc, N-acetylmannosamine monohydrate), manufactured in bulk (GMP) at NZP, is a ubiquitous but rare monosaccharide involved in a range of metabolic processes. It is often used as a precursor in the chemical or enzymatic synthesis of the neuraminic acids found in glycolipids and glycoproteins. In biological systems it is the precursor compound for the biosynthesis of the sialic acids especially N-acetylneuraminic acid (Neu5Ac, NANA). For this reason N-acetylmannosamine is of keen interest to scientists around the world. NZP's N-acetylmannosamine is top grade material suitable for all purposes, biological or synthetic.

N-Acetylmannosamine is becoming a favoured media component to enhance sialylation in CHO, BHK and HEK mammalian cell culture glycoprotein and mAb production.

Use of Chemical

N-Acetyl-D-mannosamine (ManNAc) is used for the synthesis of sialic (N-acetylneuraminic) acid. It is also a synthetic intermediate for a number of carbohydrate-derived families of biologically active compounds and pharmaceutical candidates.


  • CAS Number: 676347-48-1; (7772-94-3; 3615-17-6)
  • Molecular Weight: 221.2 (Anhydrous)
  • Solubility: Water soluble
  • Packaging: N-Acetyl-D-mannosamine is a white powder that is packaged and sealed in two polyethylene liners and packed in 10 kg plastic pails. Customer specific packaging can be provided.

Relevant Publications

Quantitative hydrophilic interaction chromatography–mass spectrometry analysis of N-acetylneuraminic acid and N-acetylmannosamine in human plasma
Yifan Shi, Xin Xu, Meng Fang, Michael Zhang, Yinghe Li, Brad Gillespie, Selwyn Yorke, Nora Yang, John C. McKew, William A Gahl, Marjan Huizing, Nuria Carrillo-Carrasco, Amy Qiu Wang


• HILIC LC MS/MS methods for ManNAc and Neu5Ac in human plasma are described.
• A fit-for-purpose approach was adopted during method validation and sample analysis.
• A surrogate matrix, 5% BSA, was utilized for the preparation of calibration standards.
• Extraction with a phospholipid removal plate eliminated matrix effect.

Read the full article here.

"N-Acetylmannosamine and Mammalian Cell Culture Production of Recombinant Therapeutic Human Glycoproteins"

This expanded update of the mini-review originally published early in 2013, covers developments in the understanding, role and value of N-acetylmannosamine (ManNAc) to increase sialylation in the production of recombinant therapeutic human glycoproteins and monoclonal antibodies.

Read the review here

"The application of N-acetylmannosamine to the mammalian cell culture production of recombinant human glycoproteins."

This mini-review covers recent developments in the understanding, role and value of N-acetylmannosamine (ManNAc) as a compound that can improve sialylation during the production of recombinant human glycoproteins.

Read the review here

 "Effects of Sugar Chain Precursors on Recombinant Protein Production in BHK Cells". Megumi Hayashi,  Kaori Doi,  Ichiro Ebata,  Shinya Yamaguchi,  Yasuhiro Ohta,  Satoshi Terada, Animal Cell Technology: Basic & Applied Aspects Volume 15,  2009,   pp 81-86.

“Improvement of interferon-gamma sialylation in Chinese hamster ovary cell culture by feeding of N-acetylmannosamine”. Gu X, Wang DI. Biotechnol Bioeng. 1998 Jun 20;58(6):642-8.